The 75 µm × 25 cm column provides superior resolution thanks to its longer length, which enables more efficient separation of complex mixtures. However, the longer column results in lower throughput. It is the best choice when maximizing resolution is the priority, even at the cost of sample analysis time.
Performance Overview
Sensitivity: ⭐⭐⭐ High
Resolution: ⭐⭐⭐⭐ Very High
Throughput: ⭐⭐ Moderate
Typical Use Case: Applications requiring high resolution and better separation, such as complex proteomics or metabolomics samples