Columns

 Column Quality Control

The traces below show the performance of a typical 50µm ID x 150mm 1.7µm 100Å MicrOmics C18 packed tip column run at 90 nl/min from 5-35% ACN/0.1% Formic Acid over 20 minutes for a 2.0 ng HeLa tryptic digest standard. The lower trace shows an average peak width at half height of 3 seconds, resulting in a peak capacity of ~400, which is over twice the resolution of comparable sub 2 micron particle columns (J. Proteome Res. 2022, 21, 2846−2892 - see graphic below).

 

 

Packed Tip Outlet

MicrOmics has developed a unique method of tip preparation based on Dr. Ryan Kelly's years of experience in designing ESI emitters at the Pacific Northwest National Labs and Brigham Young University.  This technology allows us to create robust and reproducible performance for packed tip columns from 25-75µm ID at flow rates from 25 - 500 nl/min.